Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia

Program
biccn
Collection Type
Static

"The human cortex comprises diverse cell types that emerge from an initially uniform neuroepithelium that gives rise to radial glia, the neural stem cells of the cortex. To characterize the earliest stages of human brain development, we performed single-cell RNA-sequencing across regions of the developing human brain, including the telencephalon, diencephalon, midbrain, hindbrain and cerebellum. We identify nine progenitor populations physically proximal to the telencephalon, suggesting more heterogeneity than previously described, including a highly prevalent mesenchymal-like population that disappears once neurogenesis begins. Comparison of human and mouse progenitor populations at corresponding stages identifies two progenitor clusters that are enriched in the early stages of human cortical development. We also find that organoid systems display low fidelity to neuroepithelial and early radial glia cell types, but improve as neurogenesis progresses. Overall, we provide a comprehensive molecular and spatial atlas of early stages of human brain and cortical development."

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restricted
License
CC BY 4.0

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  • Download BDBag https://data.nemoarchive.org/publication_release/Kriegstein_Early_Human_Development_Radial_Glia_2021_bdbag_2021_06_04.tgz 62 files 1.18 GB
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No anatomical region listed for Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia collection.

Taxa

human

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Assays, Modalities & Techniques

Assays
No assays listed
Modalities
transcriptome
Techniques
10x chromium 3' v2 sequencing

Child collections

  • nemo:dat-mkta0nw
    Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia: Processed data
  • nemo:dat-9pjx0f7
    Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia: Raw data

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Associated publications

  • Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia https://doi.org/10.1038/s41593-020-00794-1

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