| Field |
Value |
| Identifier |
nemo:dat-0rsydy7 |
| Dataset Name |
Single-Cell Atlas of Early Human Brain Development Highlights Heterogeneity of Human Neuroepithelial Cells and Early Radial Glia |
| Version |
1 |
| Release Date |
NA |
| DOI |
NA |
| Source Data URL |
|
| Dataset Collection URL |
https://data.nemoarchive.org/publication_release/Kriegstein_Early_Human_Development_Radial_Glia_2021_bdbag_2021_06_04.tgz |
| Description |
The human cortex is comprised of diverse cell types that emerge from an initially uniform neuroepithelium that gives rise to radial glia, the neural stem cells of the cortex. To characterize the earliest stages of human brain development, we performed single-cell RNA-sequencing across regions of the developing human brain, including the telencephalon, diencephalon, midbrain, hindbrain, and cerebellum. We identify nine progenitor populations physically proximal to the telencephalon, suggesting more heterogeneity than previously described, including a highly prevalent mesenchymal-like population that disappears once neurogenesis begins. Comparison of human and mouse progenitor populations at corresponding stages identifies two progenitor clusters that are enriched in the early stages of human cortical development. We also find that organoid systems display low fidelity to neuroepithelial and early radial glia cell types, but improve as neurogenesis progresses. Overall, we provide a comprehensive molecular and spatial atlas of early stages of human brain and cortical development. |
| Keywords |
|
| Total Files in Collection |
0 |
| Total Size in Collection (in GB) |
0.0 |
| Authors |
Ugomma C. Eze, Aparna Bhaduri, Maximilian Haeussler, Tomasz J. Nowakowski, Arnold R. Kriegstein |
| Organization |
Department of Neurology, University of California, San Francisco (UCSF); The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco (UCSF); Genomics Institute, University of California, Santa Cruz; Department of Anatomy, University of California, San Francisco (UCSF) |
| Contact Person |
Aparna Bhaduri, Arnold R. Kriegstein |
| Contact E-Mail |
ABhaduri@mednet.ucla.edu, Arnold.Kriegstein@ucsf.edu |
| External Identifier |
https://doi.org/10.1101/2020.03.06.981423 |
| Grant Name |
This study was supported by NIH award U01MH114825 A.R.K., and F32NS103266, K99NS111731, and L'Oreal For Women in Science Award through the AAAS to A.B. |
| Consortium |
BICCN |
| Data Repository |
NeMO |
| Data Repository RRID |
RRID:SCR_016152 |
| Data License |
CC BY 4.0 |
| Data Access |
https://biccn.org/terms-of-use |
| Community Standards |
https://biccn.org/standards |
| Study Organism |
Human (Homo sapiens), Mouse (Mus musculus) |
| Protocol ID |
None |
