| Field |
Value |
| Identifier |
nemo:dat-76h044v |
| Dataset Name |
A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons |
| Version |
NA |
| Release Date |
NA |
| DOI |
NA |
| Source Data URL |
NA |
| Dataset Collection URL |
https://data.nemoarchive.org/publication_release/Zeng_He_sn_RNAseq_SPN_2023.tgz |
| Description |
The brain controls nearly all bodily functions via spinal projecting neurons (SPNs) that carry command signals from the brain to the spinal cord. However, a comprehensive molecular characterization of brain-wide SPNs is still lacking. Here, we transcriptionally profiled a total of 65,002 SPNs, identified 76 region-specific SPN types, and mapped these types into a companion atlas of the whole mouse brain. This taxonomy reveals a three-component organization of SPNs: (1) molecularly homogeneous excitatory SPNs from the cortex, red nucleus, and cerebellum with somatotopic spinal terminations suitable for point-to-point communication; (2) heterogeneous populations in the reticular formation with broad spinal termination patterns, suitable for relaying commands related to the activities of the entire spinal cord; and (3) modulatory neurons expressing slow-acting neurotransmitters and/or neuropeptides in the hypothalamus, midbrain, and reticular formation for gain control of brain-spinal signals. Further, this atlas revealed a LIM homeobox transcription factor code that parcellates the reticulospinal neurons into five molecularly distinct and spatially segregated populations.Finally, we found transcriptional signatures of a subset of SPNs with large soma size and correlated these with fast-firing electrophysiological properties. Together, this study establishes a comprehensive taxonomy of brain-wide SPNs and provides insight into the functional organization of SPNs in mediating brain control of bodily functions. |
| Keywords |
single nucleus RNA-seq, transcriptome, mouse, spinal projecting neuron, corticospinal, rubrospinal, reticulospinal |
| Total Files in Collection |
4453 |
| Total Size in Collection (in GB) |
818.0 |
| Authors |
Carla C. Winter, Anne Jacobi, Junfeng Su, Leeyup Chung, Cindy T. J. van Velthoven, Zizhen Yao, Changkyu Lee, Zicong Zhang, Shuguang Yu, Kun Gao, Geraldine Duque Salazar, Evgenii Kegeles, Yu Zhang, Makenzie C. Tomihiro, Yiming Zhang, Zhiyun Yang, Junjie Zhu, Jing Tang, Xuan Song, Ryan J. Donahue, Qing Wang, Delissa McMillen, Michael Kunst, Ning Wang, Kimberly A. Smith, Gabriel E. Romero, Michelle M. Frank, Alexandra Krol, Riki Kawaguchi, Daniel H. Geschwind, Guoping Feng, Lisa V. Goodrich, Yuanyuan Liu, Bosiljka Tasic, Hongkui Zeng, Zhigang He |
| Organization |
Boston Children's Hospital, Harvard Medical School, Allen Institute for Brain Science |
| Contact Person |
Carla C. Winter, Hongkui Zeng, Zhigang He |
| Contact E-Mail |
carla_winter@hms.harvard.edu, hongkuiz@alleninstitute.org, zhigang.he@childrens.harvard.edu |
| External Identifier |
NA |
| Grant Name |
This work was supported by the National Institutes of Health (F30AG074598, U19MH114830, R01NS109947 and R01AT010779); Allen Institute for Brain Science; Wings for Life Spinal Cord Research Foundation; Department of Public Health of Massachusetts; Dr. Miriam and Sheldon G. Adelson Medical Research Foundation |
| Consortium |
BICCN |
| Data Repository |
NeMO |
| Data Repository RRID |
RRID:SCR_016152 |
| Data License |
CC BY 4.0 |
| Data Access |
https://biccn.org/terms-of-use |
| Community Standards |
https://biccn.org/standards |
| Study Organism |
Mus musculus |
| Protocol ID |
dx.doi.org/10.17504/protocols.io.bq6wmzfe; dx.doi.org/10.17504/protocols.io.bq7emzje; dx.doi.org/10.17504/protocols.io.8epv517xdl1b/v2 |
