Identifiers

Identifier Results

Field Value
Identifier nemo:dat-7qjdj84
Dataset Name Enhancer viruses for combinatorial cell-subclass-specific labeling
Version NA
Release Date NA
DOI NA
Source Data URL
Dataset Collection URL
Description Rapid cell type identification by new genomic single-cell analysis methods has not been met with efficient experimental access to these cell types. To facilitate access to specific neural populations in mouse cortex, we collected chromatin accessibility data from individual cells and identified enhancers specific for cell subclasses and types. When cloned into recombinant adeno-associated viruses (AAVs) and delivered to the brain, these enhancers drive transgene expression in specific cortical cell subclasses. We extensively characterized several enhancer AAVs to show that they label different projection neuron subclasses as well as a homologous neuron subclass in human cortical slices. We also show how coupling enhancer viruses expressing recombinases to a newly generated transgenic mouse, Ai213, enables strong labeling of three different neuronal classes/subclasses in the brain of a single transgenic animal. This approach combines unprecedented flexibility with specificity for investigation of cell types in the mouse brain and beyond.
Keywords mouse; ATAC-seq; cortex
Total Files in Collection 0
Total Size in Collection (in GB) 0.0
Authors Graybuck, L.T., Daigle, T.L., Sedeño-Cortés, A.E. et al.
Organization Allen Institute for Brain Science
Contact Person Bosiljka Tasic
Contact E-Mail bosiljkat@alleninstitute.org
External Identifier
Grant Name R01DA036909; RF1MH121274; RF1MH114126
Consortium BICCN
Data Repository NeMO
Data Repository RRID RRID:SCR_016152
Data License CC BY 4.0
Data Access
Community Standards
Study Organism mouse
Protocol ID dx.doi.org/10.17504/protocols.io.bq6wmzfe; dx.doi.org/10.17504/protocols.io.be4cjgsw; dx.doi.org/10.17504/protocols.io.7d6hi9e
Field Value
Identifier
Description scRNA-Seq data associated with the publication 'Enhancer viruses for combinatorial cell-subclass-specific labeling'
Source Data URL
Field Value
Identifier
Description scATAC-Seq data associated with the publication 'Enhancer viruses for combinatorial cell-subclass-specific labeling'
Source Data URL

This identifier does not have sub-identifiers with "analysis" data associated

HOWTO: Download BDBag Data