Identifier Results
| Field | Value |
|---|---|
| Identifier | nemo:dat-7uhwm74 |
| Dataset Name | Integrated Morphoelectric and Transcriptomic Classification of Cortical GABAergic Cells |
| Version | NA |
| Release Date | NA |
| DOI | NA |
| Source Data URL | http://data.nemoarchive.org/other/AIBS/AIBS_patchseq/transcriptome/scell/SMARTseq/ |
| Dataset Collection URL | https://data.nemoarchive.org/publication_release/MousePSeqVIS_T.tgz |
| Description | Neurons are frequently classified into distinct types on the basis of structural, physiological, or genetic attributes. To better constrain the definition of neuronal cell types, we characterized the transcriptomes and intrinsic physiological properties of over 4,200 mouse visual cortical GABAergic interneurons and reconstructed the local morphologies of 517 of those neurons. We find that most transcriptomic types (t-types) occupy specific laminar positions within visual cortex, and, for most types, the cells mapping to a t-type exhibit consistent electrophysiological and morphological properties. These properties display both discrete and continuous variation among t-types. Through multimodal integrated analysis, we define 28 met-types that have congruent morphological, electrophysiological, and transcriptomic properties and robust mutual predictability. We identify layer-specific axon innervation pattern as a defining feature distinguishing different met-types. These met-types represent a unified definition of cortical GABAergic interneuron types, providing a systematic framework to capture existing knowledge and bridge future analyses across different modalities. |
| Keywords | GABAergic interneurons, somatostatin, parvalbumin, neuronal cell type, transcriptomics, taxonomy, Patch-seq, multimodal, visual cortex |
| Total Files in Collection | 8879 |
| Total Size in Collection (in GB) | 830.5 |
| Authors | Nathan W. Gouwens, Staci A. Sorensen, Fahimeh Baftizadeh, Agata Budzillo, Brian R. Lee, Tim Jarsky, Lauren Alfiler, Katherine Baker, Eliza Barkan, Kyla Berry, Darren Bertagnolli, Kris Bickley, Jasmine Bomben, Thomas Braun, Krissy Brouner, Tamara Casper, Kirsten Crichton, Tanya L. Daigle, Rachel Dalley, Rebecca A. de Frates, Nick Dee, Tsega Desta, Samuel Dingman Lee, Nadezhda Dotson, Tom Egdorf, Lauren Ellingwood, Rachel Enstrom, Luke Esposito, Colin Farrell, David Feng, Olivia Fong, Rohan Gala, Clare Gamlin, Amanda Gary, Alexandra Glandon, Jeff Goldy, Melissa Gorham, Lucas Graybuck, Hong Gu, Kristen Hadley, Michael J. Hawrylycz, Alex M. Henry, DiJon Hill, Madie Hupp, Sara Kebede, Tae Kyung Kim, Lisa Kim, Matthew Kroll, Changkyu Lee, Katherine E. Link, Matthew Mallory, Rusty Mann, Michelle Maxwell, Medea McGraw, Delissa McMillen, Alice Mukora, Lindsay Ng, Lydia Ng, Kiet Ngo, Philip R. Nicovich, Aaron Oldre, Daniel Park, Hanchuan Peng, Osnat Penn, Thanh Pham, Alice Pom, Zoran Popović, Lydia Potekhina, Ramkumar Rajanbabu, Shea Ransford, David Reid, Christine Rimorin, Miranda Robertson, Kara Ronellenfitch, Augustin Ruiz, David Sandman, Kimberly Smith, Josef Sulc, Susan M. Sunkin, Aaron Szafer, Michael Tieu, Amy Torkelson, Jessica Trinh, Herman Tung, Wayne Wakeman, Katelyn Ward, Grace Williams, Zhi Zhou, Jonathan T. Ting, Anton Arkhipov, Uygar Sümbül, Ed S. Lein, Christof Koch, Zizhen Yao, Bosiljka Tasic, Jim Berg, Gabe J. Murphy, Hongkui Zeng |
| Organization | Allen Institute for Brain Science |
| Contact Person | Gabe J. Murphy |
| Contact E-Mail | gabem@alleninstitute.org |
| External Identifier | https://doi.org/10.1016/j.cell.2020.09.057 |
| Grant Name | Allen Institute for Brain Science |
| Consortium | BICCN |
| Data Repository | NeMO |
| Data Repository RRID | RRID:SCR_016152 |
| Data License | CC BY 4.0 |
| Data Access | https://biccn.org/terms-of-use |
| Community Standards | https://biccn.org/standards |
| Study Organism | mouse |
| Protocol ID | NA |
| Field | Value |
|---|---|
| Identifier | nemo:dat-m2kd56v |
| Description | Raw Patch-Seq data from mouse associated with the following publication: Gouwens et al, 2020. Integrated Morphoelectric and Transcriptomic Classification of Cortical GABAergic Cells. Cell doi.org/10.1016/j.cell.2020.09.057 |
| Source Data URL | https://data.nemoarchive.org/other/AIBS/AIBS_patchseq/transcriptome/scell/SMARTseq/raw/20200611/ |
| Field | Value |
|---|---|
| Identifier | nemo:dat-nqrnka9 |
| Description | Analysis bag contains processed data, alignment and summary files, associated with the following publication: Gouwens et al, 2020. Integrated Morphoelectric and Transcriptomic Classification of Cortical GABAergic Cells. Cell doi.org/10.1016/j.cell.2020.09.057 |
| Source Data URL | https://data.nemoarchive.org/other/AIBS/AIBS_patchseq/transcriptome/scell/SMARTseq/processed/align/20200611/ |