Single-cell genomics reveals region-specific developmental trajectories underlying neuronal diversity in the human hypothalamus

Program
biccn
Collection Type
Static

"The development and diversity of neuronal subtypes in the human hypothalamus has been insufficiently characterized. We sequenced the transcriptomes of 40,927 cells from the prenatal human hypothalamus spanning from 6 to 25 gestational weeks and 25,424 mature neurons in regions of the adult human hypothalamus, revealing a temporal trajectory from proliferative stem cell populations to mature neurons and glia. Developing hypothalamic neurons followed branching trajectories leading to 170 transcriptionally distinct neuronal subtypes in ten hypothalamic nuclei in the adult. The uniqueness of hypothalamic neuronal lineages was examined developmentally by comparing excitatory lineages present in cortex and inhibitory lineages in ganglionic eminence from the same individuals, revealing both distinct and shared drivers of neuronal maturation across the human forebrain. Cross-species comparisons to the mouse hypothalamus identified human-specific POMC populations expressing unique combinations of transcription factors and neuropeptides. These results provide the first comprehensive transcriptomic view of human hypothalamus development at cellular resolution."

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Access
restricted
License
Public data available under CC BY 4.0; controlled access data subject to data use certification

Download URLs

  • Download BDBag https://data.nemoarchive.org/publication_release/Herb_SAment_Transcriptomic_Hypothalamus_2023.tgz 367 files 1,145.53 GB
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Anatomical Regions

No anatomical region listed for Single-cell genomics reveals region-specific developmental trajectories underlying neuronal diversity in the human hypothalamus collection.

Taxa

human

Projects this collection belongs to

  • nemo:std-gnk4c0r
    Transcriptomic characterization of cell types in human brain: 10x nuclei v2 and v3

Assays, Modalities & Techniques

Assays
No assays listed
Modalities
transcriptome
Techniques
10x chromium 3' v2 sequencing 10x chromium 3' v3 sequencing

Child collections

  • nemo:dat-mdrshp9
    Single-cell genomics reveals region-specific developmental trajectories underlying neuronal diversity in the human hypothalamus: 10x_Raw data
  • nemo:dat-jzpa6m9
    Single-cell genomics reveals region-specific developmental trajectories underlying neuronal diversity in the human hypothalamus: 10x_Analysis

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Associated publications

  • Single-cell genomics reveals region-specific developmental trajectories underlying neuronal diversity in the human hypothalamus https://doi.org/10.1101/2021.07.20.453090

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Documentation & resources