Identifier Results
| Field | Value |
|---|---|
| Identifier | nemo:dat-hsgdsgu |
| Dataset Name | Marmosets have their birth siblings’ microglia |
| Version | NA |
| Release Date | NA |
| DOI | NA |
| Source Data URL | NA |
| Dataset Collection URL | NA |
| Description | Chimerism is the presence in the same animal of cells from multiple animals. Marmosets, which most frequently develop as fraternal twins or triplets with circulatory systems that are connected in utero, harbor persistent chimerism in the blood. The presence of Y-chromosome DNA sequences in the organs of female marmosets with male birth siblings has long suggested that other organs might also exhibit chimerism, but whether this arises from blood or other cell types has been unknown. Here we show by single-cell RNA-seq that, in the liver and kidney, chimerism arises from infiltrating macrophages and other blood-derived monocytes. Intriguingly, though, the marmoset brain’s microglia consist of genetically distinct populations: one with the animal’s own genome, and other population(s) with the genomes of the animal’s birth sibling(s). Across 137 tissue samples from 11 animals, cells from birth siblings comprised 20-52% of an animal’s microglia. Sibling microglial populations inhabited different brain areas in distinct relative proportions, suggesting that microglia with sibling genomes were differentially responsive to local recruitment or proliferation cues, or had expanded clonally. Sibling microglia exhibited clear gene-expression differences, but analyses of siblings’ microglia in both siblings’ brains indicated that context (host brain) played a larger role in shaping their gene expression than genomic differences did. Chimerism will offer powerful, well-controlled ways to study the effects of genes, mutations and brain contexts on microglial biology and to distinguish between effects of microglia and other cell types on brain phenotypes. |
| Keywords | marmoset, chimerism, microglia, cortex, thalamus, striatum, hippocampus, basal forebrain, hypothalamus, amygdala, cerebellum |
| Total Files in Collection | 0 |
| Total Size in Collection (in GB) | 0.0 |
| Authors | Ricardo C.H. del Rosario, Fenna M. Krienen, Qiangge Zhang, Melissa Goldman, Curtis Mello, Kiku Ichihara, Alyssa Lutservitz, Alec Wysoker, James Nemesh, Guoping Feng, Steven A. McCarroll |
| Organization | Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard; Department of Genetics, Harvard Medical School; McGovern Institute for Brain Research, Massachusetts Institute of Technology |
| Contact Person | Ricardo del Rosario |
| Contact E-Mail | rcdelros@broadinstitute.org |
| External Identifier | NA |
| Grant Name | Broad Institute’s Stanley Center for Psychiatric Research |
| Consortium | BICCN |
| Data Repository | NeMO |
| Data Repository RRID | RRID:SCR_016152 |
| Data License | CC BY 4.0 |
| Data Access | https://biccn.org/terms-of-use |
| Community Standards | https://biccn.org/standards |
| Study Organism | common marmoset (Callithrix jacchus) |
| Protocol ID | dx.doi.org/10.17504/protocols.io.2srged6 |
This identifier does not have sub-identifiers with "raw" data associated
This identifier does not have sub-identifiers with "analysis" data associated