Transcriptomic cell types in the mouse brain: 10x v2 nuclei

"Our goal is to create a high-quality atlas of cell types in the mouse brain using large scale single cell transcriptomic analysis across the entire mouse brain. Coarse dissections of brain regions were performed and specimens were dissected from both hemispheres of male and female mice. Nuclei were extracted from dissected tissues, and fluorescence activated sorting of nuclei was performed based on antibody labeling of NeuN protein to obtains pools of nuclei enriched for neurons. These pools of nuclei were processed for single nucleus RNA sequencing using 10X Chromium v2, which isolated nuclei in droplets to assign individual nucleus barcodes. This method enables a rapid, large-scale survey of cell types in brain regions with accurate recovery of cell type proportions, but at low gene coverage per nucleus. This dataset surveys cells in the primary motor cortex (MOp) as well as regions where isolation of whole cells is difficult, or nuclear profiling is needed to establish cell type proportions."

Data citation

Dataset: Hongkui Zeng, Cindy Van Velthoven, Bosiljka Tasic (2021) Transcriptomic cell types in the mouse brain: 10x v2 nuclei [Dataset]. Available from: https://assets.nemoarchive.org/dat-zsm81og

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CC BY 4.0