DNA methylation profiling of genomic DNA in individual mouse brain cell nuclei (RS1.1)

Project at a glance

Program

biccn

Submission status

['cell nucleus']

Funding support

Not listed

Consortium RRID

Not listed

Organizations

Salk Institute for Biological Studies, University of California, Los Angeles, University of California, San Diego

Scope

Taxa

No taxa listed

Modalities

epigenome transcriptome multimodal

Techniques

mc-seq2 10x chromium 3' v2 sequencing 10x chromium 3' v3 sequencing atac-seq smart-seq v4

Assays

No assays listed

License

None, CC BY 4.0

Description

"The goal is to generate single nucleus methyl-C genomic DNA sequencing data from isolated cells from >100 regions of the mouse brain for both cell-type classification and the identification of regulatory elements. The brains of adult male C57BL/6J mice (P56) were sliced into 18 600-micron-thick coronal sections and tissues isolated from selected regions in each slice were pooled separately (6-30 animals per region, hemispheres combined). NeuN-positive and NeuN-negative cell nuclei were purified from each pool, and isolated nuclei subjected to single nuclei methyl-C DNA sequencing (snmCseq2). (Note: The same tissue pools were used in both RS1.1 and RS1.2.) Over 100 selected brain regions were sampled across both brain hemispheres in male mice."

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