Human Multiple Cortical Areas - SMART-seq

Project at a glance

Program

other

Submission status

['cell nucleus']

Funding support

Not listed

Consortium RRID

Not listed

Organizations

Allen Institute

Scope

Taxa

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Modalities

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Techniques

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Assays

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Description

"To investigate the cellular diversity across human cortex, we used SMART-Seq v4 single nucleus RNA-sequencing that provides a relatively unbiased survey of cell types and can be applied to neurosurgical and frozen, postmortem tissue. Individual layers of cortex were dissected from brain tissues including the middle temporal gyrus (MTG), anterior cingulate gyrus (CgGr), primary visual cortex (V1C), primary motor cortex (M1C), primary somatosensory cortex (S1C) and primary auditory cortex (A1C), and nuclei were dissociated and sorted using the neuronal marker NeuN. In total, nuclei from MTG were sampled from four postmortem donor brains and four neurosurgical donor brains and nuclei from the remaining regions were sampled from 3 postmortem donor brains. Profiled nuclei included approximately 90% neurons and 10% glia. NOTE: This project contains a mixture of datasets generated under different funding sources and associated with different access restrictions and licenses. Please refer to the description of the data collection to identify which license is applicable."

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