Identifier Results
| Field | Value |
|---|---|
| Identifier | nemo:dat-1je0mn3 |
| Dataset Name | A marmoset brain cell census reveals persistent influence of developmental origin on neurons |
| Version | NA |
| Release Date | NA |
| DOI | NA |
| Source Data URL | https://data.nemoarchive.org/biccn/grant/u01_feng/mccarroll/ |
| Dataset Collection URL | NA |
| Description | Within the vertebrate neocortex and other telencephalic structures, molecularly-defined neurons tend to segregate at first order into inhibitory (GABAergic) and excitatory (glutamatergic) types. We used single-nucleus RNA sequencing, analyzing over 2.4 million brain cells sampled from 16 locations in a primate (the common marmoset) to ask whether (1) neurons generally segregate by neurotransmitter status, and (2) neurons expressing the same neurotransmitters share additional molecular features in common, beyond the few genes directly responsible for neurotransmitter synthesis and release. We find the answer to both is “no”: there is a remarkable degree of transcriptional similarity between GABAergic and glutamatergic neurons found in the same brain structure, and there is generally little in common between glutamatergic neurons residing in phylogenetically divergent brain structures. The origin effect is permanent: we find that cell types that cross cephalic boundaries in development retain the transcriptional identities of their birthplaces. GABAergic interneurons, which migrate widely, follow highly specialized and distinct distributions in striatum and neocortex. We use interneuron-restricted AAVs to reveal the morphological diversity of molecularly defined types. Our analyses expose how lineage and functional class sculpt the transcriptional identity and biodistribution of primate neurons. |
| Keywords | snRNA-seq, 10x Genomics 3’ v3.1 |
| Total Files in Collection | 0 |
| Total Size in Collection (in GB) | 0.0 |
| Authors | Fenna M. Krienen, Kirsten M. Levandowski, Heather Zaniewski, Ricardo C.H. del Rosario, Margaret E. Schroeder, Melissa Goldman, Alyssa Lutservitz, Qiangge Zhang, Katelyn X. Li, Victoria F. Beja-Glasser, Jitendra Sharma, Tay Won Shin, Abigail Mauermann, Alec Wysoker, James Nemesh, Seva Kashin, Josselyn Vergara, Gabriele Chelini, Jordane Dimidschstein, Sabina Berretta, Ed Boyden, Steven A. McCarroll, Guoping Feng |
| Organization | Harvard Medical School, Stanley Center for Psychiatric Research, McGovern Institute for Brain Research |
| Contact Person | Fenna M. Krienen, Steven A. McCarroll, Guoping Feng |
| Contact E-Mail | krienen@broadinstitute.org, mccarroll@genetics.med.harvard.edu, fengg@mit.edu |
| External Identifier | https://doi.org/10.1101/2022.10.18.512442 |
| Grant Name | 1U01MH114819-01 |
| Consortium | BICCN |
| Data Repository | NeMO |
| Data Repository RRID | RRID:SCR_016152 |
| Data License | CC BY 4.0 |
| Data Access | https://biccn.org/terms-of-use |
| Community Standards | https://biccn.org/standards |
| Study Organism | marmoset |
| Protocol ID | NA |
This identifier does not have sub-identifiers with "raw" data associated
This identifier does not have sub-identifiers with "analysis" data associated