Structure-function cell atlas for norepinephrine and serotonin neurons

Grant at a glance

Short name

BICAN_Neuromodulation

Grant number

R01MH134833

Funding agency

NIMH

Program

bican

Description

Norepinephrine (NE) and serotonin (5-HT) are neurotransmitters released by a small number of neurons in the locus coeruleus (LC) and dorsal raphe (DR), respectively. Each group of neurons provides extensive innervation of most of the brain. Although these groups of cells are often considered to be homogenous, recent data indicates the existence of subclasses of each neuron. This proposal aims to generate multi-scale data on each system, comprising molecular, genetic, anatomical, physiological, and behavioral experiments. Our team is in a unique position to integrate across experiments and develop a new understanding of the subclasses of LC-NE and DR-5-HT neurons and their functions during behavior. Three aims test specific questions about the cell types and functions of LC-NE and DR-5-HT neurons in mice: 1) LC-NE neuron subclasses have distinct biophysical properties and axonal projections; 2) subclasses of DR-5-HT neurons have distinct biophysical properties and axonal projections; and 3) subclasses of LC-NE and DR-5-HT neurons have different functions during dynamic decision making. Our experiments will be used to develop a comprehensive classification of NE and 5-HT neuronal subclasses and will be used to generate reagents to target those cells.

Collections from this grant

Associated labs

• aibs

Methods supported

Modalities

transcriptome multimodal epigenome

Techniques

SMARTSeqSC 10X genomics multiome;atac-seq 10x Chromium 3' v4 sequencing 10X genomics multiome;rnaseq 10x genomics multiome smart-seq v4 10x chromium 3' v3 sequencing 10x chromium 3' v3.1 sequencing m3c-seq 10xV3.1_HT_CellPlex;Barcode 10xV3.1_HT 10xV3.1_HT_CellPlex;GEX 10xV3.1_CellPlex;Barcode 10xV3.1_CellPlex;GEX droplet paired-tag; DNA droplet paired-tag; RNA multiplex droplet paired-tag; RNA 10x chromium 5' v3 sequencing 10x chromium 5' v2 sequencing snmCT-seq 10x Chromium 3' v4-OCM sequencing 10xV3.1_LT

Assays

transcriptome multiome; chromatin multiome; transcriptome methylation HT; transcriptome transciptome; cellplex_barcode transciptome; cellplex_RNAseq HT; cellplex_barcode HT; cellplex_RNAseq multimodal LT; transcriptome

Taxa