Functionally guided adult whole brain cell atlas in human and NHP

Grant at a glance

Short name

HMBA

Grant number

UM1MH130981

Funding agency

NIMH

Program

bican

Description

The Human and Mammalian Brain Atlas (HMBA) consortium aims to create new whole brain human and non-human primate cell atlases linked to functional brain architecture. Single cell transcriptomic, epigenomic and spatial transcriptomics will be used to classify and spatially map cell types across the entire human, macaque and marmoset brain, sampling based on brain maps derived from structural and functional imaging, with additional targeted analyses to understand variation across human individuals and a broader range of mammalian species. Molecularly defined cell types will be characterized for cellular anatomy and physiology, using enhancer-AAV based tools to allow selective genetic labeling of cell types where possible. The outcome of these efforts will produce a new reference classification for cell types across the whole human and NHP brain, spatial maps of molecularly defined cell types, and phenotypic characterization of fundamental brain cell types, all aligned in common coordinate frameworks. This effort will create new multiscale, high resolution brain atlases integrating structural, functional, cellular and molecular information between human and non-human primate and rodent model organisms.

Collections from this grant

Associated labs

• aibs
• princeton_lein

Methods supported

Modalities

multimodal transcriptome epigenome

Techniques

10X genomics multiome;atac-seq 10X genomics multiome;rnaseq 10xV3.1_HT_CellPlex;GEX 10xV3.1_HT_CellPlex;Barcode SMARTSeqSC 10xV3.1_CellPlex;Barcode 10xV3.1_CellPlex;GEX patch-seq 10x Chromium 3' v4-OCM sequencing 10x Chromium 3' v4 sequencing 10x chromium 3' v3.1 sequencing 10xV3.1_HT smart-seq v4 m3c-seq snmCT-seq 10xV3.1_LT 10x chromium 3' v3 sequencing droplet paired-tag; DNA droplet paired-tag; RNA multiplex droplet paired-tag; RNA 10x chromium 5' v3 sequencing 10x chromium 5' v5.2 sequencing

Assays

multiome; chromatin multiome; transcriptome transcriptome transciptome; cellplex_barcode transciptome; cellplex_RNAseq HT; cellplex_barcode HT; cellplex_RNAseq HT; transcriptome spatial transcriptome methylation multimodal LT; transcriptome

Taxa